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A) IHC staining showing CD103+/CD11c+ cells in fibrotic foci. GFPCol1a1 mice were given fibrosis via blm instillation, and lungs were fixed in 10% buffered PFA on day 21. Lungs were processed and stained for IHC against GFP, CD103, and CD11c. Arrows indicate cells with red/cyan staining. Inset: wide view. B) Additional images showing the presence of CD103+ CD11c+ DCs in fibrotic but not normal areas of lung sections obtained from mice with bleomycin induced fibrosis. C and D) iCD103 DCs were mono-cultured in complete media with 25 nM ODN2395 (ODN) or ODN + 100 μM kyn (ODN + Kyn), or co- cultured with 1.5×10 5 primary murine lung fibroblasts (MLF + DC) isolated from lung minces of mice with bleomycin induced fibrosis (21-days post bleo), MLF cells were cultured alone as control. n = 3 per group. E) iCD103 DCs were incubated with 50% BALF from mice 21-d post blm. RNA was isolated and analyzed for the indicated transcript (n = 5-6 samples per group. Statisitcal significance was assessed via ANOVA, ** = p < 0.01, **** = p < 0.001.

Journal: bioRxiv

Article Title: Cell-Specific Modulation of the Aryl Hydrocarbon Receptor by Kynurenine in Pulmonary Fibrosis Requires Microenvironmental Crosstalk

doi: 10.64898/2026.07.21.738475

Figure Lengend Snippet: A) IHC staining showing CD103+/CD11c+ cells in fibrotic foci. GFPCol1a1 mice were given fibrosis via blm instillation, and lungs were fixed in 10% buffered PFA on day 21. Lungs were processed and stained for IHC against GFP, CD103, and CD11c. Arrows indicate cells with red/cyan staining. Inset: wide view. B) Additional images showing the presence of CD103+ CD11c+ DCs in fibrotic but not normal areas of lung sections obtained from mice with bleomycin induced fibrosis. C and D) iCD103 DCs were mono-cultured in complete media with 25 nM ODN2395 (ODN) or ODN + 100 μM kyn (ODN + Kyn), or co- cultured with 1.5×10 5 primary murine lung fibroblasts (MLF + DC) isolated from lung minces of mice with bleomycin induced fibrosis (21-days post bleo), MLF cells were cultured alone as control. n = 3 per group. E) iCD103 DCs were incubated with 50% BALF from mice 21-d post blm. RNA was isolated and analyzed for the indicated transcript (n = 5-6 samples per group. Statisitcal significance was assessed via ANOVA, ** = p < 0.01, **** = p < 0.001.

Article Snippet: Primary antibodies used were: GFP (Abcam ab13970 used at 0.1mg/mL), CD103 (GeneTex GTX53154 used at 2ug/mL), and CD11c (Sysy Antibodies HS-375 003 used at 1:100 dilution).

Techniques: Immunohistochemistry, Staining, Cell Culture, Isolation, Control, Incubation

Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.

Journal: Human Mutation

Article Title: A Macrophage/Monocyte‐Related Four‐Gene Signature for Prognostic Assessment of Uveal Melanoma: BTBD6 , C2CD4B , CCL24 , and S100A4

doi: 10.1155/humu/4978880

Figure Lengend Snippet: Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.

Article Snippet: Melanoma cell line A375 (CRL‐1619) and human epidermal melanocytes (PCS‐200‐013) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia) and cultured as follows.

Techniques: Reverse Transcription, Biomarker Discovery, Knockdown, Migration, In Vitro